reverse transcription in a sentence
Examples
- cDNAs obtained from reverse transcription of mRNA population extracted from the cells in varying physiological and experimental conditions are used as immobilized probes.
- The revised hypothesis was later formulated, in part, to accommodate reverse transcription ( which both Howard Temin and David Baltimore discovered in 1970 ).
- Using Reverse transcription polymerase chain reaction and a more rigorous nested primer system they found evidence for splice variants of the ETV6-NTRK3 fusion gene.
- They also concluded from quantitative reverse transcription-PCR ( qRT-PCR ) analysis that the five consecutive genes are co-regulated by the same BCG1-calcineurim transduction pathway.
- CaMV contains a circular double-stranded DNA molecule of about 8.0 kilobases, interrupted by nicks that result from the actions of RNAse H during reverse transcription.
- This is particularly important in retroviral infections since viral integration into the host genome occurs quickly after entry and reverse transcription and is largely irreversible.
- APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
- The other major high throughput technique employed in the quantification of specific RNA molecules is quantitative PCR, after reverse transcription of the RNA to cDNA.
- APOBEC3G interferes with reverse transcription of HIV-1 independent of DNA deamination . tRNA3Lys typically binds to the HIV-1 primer binding site to initiate reverse transcription.
- The ligation step adds DNA adaptors to both ends of the small RNAs, which act as primer binding sites during reverse transcription and PCR amplification.