exclusion chromatography in a sentence
Examples
- In size exclusion chromatography, where the separation process is driven by entropy, it is not possible to increase the resolution attained by a column via temperature or solvent gradients.
- Second, proteins can be separated according to their size or molecular weight via size exclusion chromatography or by SDS-PAGE ( sodium dodecyl sulfate-polyacrylamide gel electrophoresis ) analysis.
- Gel permeation chromatography, a type of size exclusion chromatography, is an especially useful technique used to directly determine the molecular weight distribution parameters based on the polymer's hydrodynamic volume.
- The highest resolution length sorting, with length variation of < 10 %, has thus far been achieved by size exclusion chromatography ( SEC ) of DNA-dispersed carbon nanotubes ( DNA-SWNT ).
- In the context of a purification strategy, denaturing condition electrophoresis provides an improved resolution over size exclusion chromatography, but does not scale to large quantity of proteins in a sample as well as the late chromatography columns.
- The most common technique for measuring molecular mass used in modern times is a variant of high-pressure liquid chromatography ( HPLC ) known by the interchangeable terms of size exclusion chromatography ( SEC ) and gel permeation chromatography ( GPC ).
- Experimental approaches for characterizing polymers are also common, using Polymer characterization methods, such as size exclusion chromatography, Viscometry, Dynamic light scattering, and Automatic Continuous Online Monitoring of Polymerization Reactions ( ACOMP ) for determining the chemical, physical, and material properties of polymers.
- The literature associated with measurements made by MALS photometers is extensive . both in reference to batch measurements of particles / molecules and measurements following fractionation by chromatographic means such as size exclusion chromatography ( SEC ), reversed phase chromatography ( RPC ), and field flow fractionation ( FFF ).
- For example, if there is any overlap between the displacer and the protein of interest, these low molecular mass materials can be readily separated from the purified protein during post-displacement processing using standard size-based purification methods ( e . g . size exclusion chromatography, ultrafiltration ).
- For fluorescence-detection size-exclusion chromatography-based thermostability assay ( FSEC-TS ) the samples are heated in the same manner as in FastPP and CETSA and after centrifugation to clear away precipitate the supernatant is treated in the same manner as FSEC . Larger aggregates are seen in the void volume while the peak height for the protein of interest decreases when the unfolding temperature is reached.