labelled protein meaning in Hindi

Examples

1. The majority of the sensors mentioned in the previous sections consisted of labelled proteins in solution.
2. MeCAT labelled proteins are identified and relatively quantified on peptide level by MALDI-MS or ESI-MS.
3. Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules.
4. Breakable PAGE SDS-PAGE ( DPAGE, dissolvable PAGE ), two-dimensional gel electrophoresis or chromatography is used for separation of MeCAT labelled proteins.
5. Called Binder Trap Enrichment ( BTE ), ligands to a protein target are identified by trapping binding pairs ( DNA-labelled protein target and yR ligand ) in emulsion droplets during dissociation dominated kinetics.
6. The MeCAT labelled proteins can be accurately quantified by ICP-MS down to low attomol amount of analyte which is at least 2 3 orders of magnitude more sensitive than other mass spectrometry based quantification methods.
7. The assignment of the HSQC spectrum requires other experiments, ideally using triple resonance experiments with 15 N and 13 C-labelled proteins, that provide sequential connectivities between residues so that the resonances can be linked to particular residues and sequentially assigned.
8. Typically, the first experiment to be measured with an isotope-labelled protein is a 2D heteronuclear single quantum correlation ( HSQC ) spectrum, where " heteronuclear " refers to nuclei other than 1H . In theory, the heteronuclear single quantum correlation has one peak for each H bound to a heteronucleus.
9. In particular, ligand-observe nuclear magnetic resonance ( NMR ) methods such as water-ligand observed via gradient spectroscopy ( waterLOGSY ), saturation transfer difference spectroscopy ( STD-NMR ), 19 F NMR spectroscopy and isotopically-labelled proteins, surface plasmon resonance ( SPR ) and isothermal titration calorimetry ( ITC ) are routinely-used for ligand screening and for the quantification of fragment binding affinity to the target protein.