• डाइगॉक्सिजेनिन |
digoxigenin meaning in Hindi
digoxigenin sentence in HindiExamples
More: Next- The hairpin is attached to the glass surface via a digoxigenin-antidigoxigenin bond.
- The latter is a common alternative to digoxigenin, and the two are used together for labelling two genes in one sample.
- Digoxigenin is thus an all-purpose immuno-tag, and in particular a standard immunohistochemical marker for in situ hybridization.
- Typically, digoxigenin is introduced chemically ( affinities and specificity, are used in a variety of biological immuno-assays ( e . g.
- More than 70 bitter glycosides with cardiac activity, with five different aglyconees digitoxigenin, gitoxigenin, digoxigenin, diginatigenin and gitaloxigenin in the leaves act as a protection against herbivores.
- To detect 32 P ( a radioactive isotope of phosphorus incorporated into the phosphodiester bond in the probe DNA ) or Digoxigenin, which is a non-radioactive, antibody-based marker.
- Digoxigenin is a hapten, a small molecule with high antigenicity, that is used in many molecular biology applications similarly to other popular haptens such as 2, 4-Dinitrophenol, biotin, and fluorescein.
- With regard to the overall method, FISH can be performed using direct labelling fluorochromes are attached to the probes or indirect labelling the probes are labelled with biotin or digoxigenin which are then detected using fluorescently-labelled streptavidin or antibodies, respectively.
- Then, the probe that was labeled with either radio-, fluorescent-or antigen-labeled bases ( e . g ., digoxigenin ) is localized and quantified in the tissue using either autoradiography, fluorescence microscopy, or immunohistochemistry, respectively.
- :1 ) In the case of double-stranded DNA molecules ( for whole genome sequencing, or targeted sequencing ), the DNA fragment is ligated to a DNA loop at one end and a DNA fork structure, labeled with biotin and digoxigenin at the two ends.